What happened to my data. Screening a mutal stabilized reporter system.

Werner Van Belle¹ - werner@yellowcouch.org, werner.van.belle@gmail.com

1- Department for Biosystems Science and Engineering; ETH Zurich; Mattenstrasse 24, Building 1058, Basel; Switzerland

Abstract: Notch signaling is one of the most important and well conserved signaling pathways. It plays an integral role in many aspects of development such as cell proliferation, survival, growth, specification, patterning etc. And its malfunction has been linked to several cancers and to a number of human diseases. Several genes involved in the Notch signaling pathway have been identified in past decades. However, a comprehensive understanding of all the players in the pathway and their molecular mechanisms and biological outcomes during development and disease is incomplete. To this end we developed a strategy to approach a genome wide RNAi screen, based on the fact that Notch signaling is induced when the Notch receptor is blocked in an endosome. We have performed the whole genome RNAi screen in Drosophila S2 cells and identified several novel genes and components of Notch processing and trafficking through enodosomes. Several mutations in genes involved in endocytosis are implicated as tumorogenic, evidently through ectopic Notch signaling. Currently, we are validating the identified candidates and focus our research efforts on to the candidate genes which modulate the Notch signaling during receptor endocytosis.

Keywords: notch, screening, iontensity dependent confidence intervals, quantile normalization
Reference: Werner Van Belle; What happened to my data. Screening a mutal stabilized reporter system.; Presented at ETH Zurich, Department of Biosystems Science and Engineering, Research Seminars; Switzerland; August 2008
See also: A poster providing some background information, All analysis graphs

http://werner.yellowcouch.org/
mailto:werner@yellowcouch.org